Vitamin D 25-Hydroxy
Also called: 25(OH)D, Vitamin D Test, Vitamin D3
Measures stored Vitamin D — the most accurate way to check for deficiency.
- Duration
- 5 min
- Report
- 48 hours
- Sample
- Blood
- Fasting
- Not needed
Save more — get this in the Comprehensive Health Checkup
13 tests · 62 parameters · ₹2,499
What this test is
25-Hydroxy Vitamin D is the form your body stores and the best single indicator of your Vitamin D status. Deficiency is extremely common across urban India even in sunny cities — most adults working indoors test low. Levels under 20 ng/mL are deficient; 20–30 are insufficient; 30–60 are adequate. Symptoms of deficiency include bone pain, muscle weakness, frequent infections, and low mood. Supplementation is cheap and effective once levels are confirmed.
When you need this test
- Bone or muscle pain
- Frequent infections or low immunity
- Persistent fatigue or low mood
- Indoor-job adults — annual screening
- Pregnancy
- Pre-menopausal and post-menopausal women
- Children with poor growth
How to prepare
- No fasting needed.
- If you take Vitamin D supplements, mention your dose and how long you've been on it.
- Avoid biotin supplements for 48 hours — they can interfere.
Why DiagnoHouse for this test
NABL-accredited lab
Quality-audited blood, urine, and stool analysis.
Home collection
Free across Pimpri-Chinchwad. Sample picked up at your time slot.
Same-day reports
Most pathology reports ready within 4 hours of collection.
Calibrated equipment
Analysers calibrated daily; quality controls run with every batch.
Patients who took this also booked
- Pathology33% OFF
Vitamin B12
Checks B12 levels — low B12 is common in vegetarians and causes nerve and energy problems.
- Duration
- 5 min
- Report time
- 24 hr report
₹1,200₹800Book this scan - Pathology28% OFF
Calcium (Total)
Standard calcium level — checks bone health, parathyroid function, and kidney status.
- Duration
- 5 min
- Report time
- 4 hr report
₹250₹180Book this scan - Pathology39% OFF
Thyroid Profile (T3, T4, TSH)
Standard three-marker thyroid panel — screens for under-active or over-active thyroid.
- Duration
- 5 min
- Report time
- 24 hr report
₹900₹550Book this scan
FAQs
What level should I aim for?
Most physicians target 40–60 ng/mL — comfortably in the adequate range with a buffer.How long does treatment take?
An 8-week high-dose course (60,000 IU once a week) usually restores levels, followed by a maintenance dose.How often should I retest?
3 months after starting supplements, then annually if stable.Is sunlight enough?
20 minutes of midday sun on arms and face, 3–4 times a week, helps — but most office-going Indians still come up deficient. Supplements are often needed.Why is the test so expensive?
It uses a specialised technique (chemiluminescence). We've priced it 33% below MRP at ₹1,200.
Need help choosing?
Not sure if this is the right test for you? Have a doctor's note? Call or WhatsApp — guidance is free.
5,000+ scans · 4.8 ★ avg rating · NABL accredited · Reports on WhatsApp